طرز تهیه EDTA نیم مولار

An EDTA (ethylenediamine tetraacetic acid) solution is prepared ahead of time. EDTA will not go completely into solution until the pH is adjusted to about 8.0. For a 500 mL stock solution of 0.5 M EDTA, weigh out 93.05 g EDTA disodium salt (FW = 372.2). Dissolve in 400 mL deionized water and adjust the pH with NaOH. Top up the solution to a final volume of 500 mL.

طرز تهیه TAE ده برابر(10x)

لینکهای زیر طرز تهیه TAE و EDTA

http://www.lifescience.net/protocols/15/10x-tae-buffer-10x-tris-acetate-edta/

http://biotech.about.com/od/buffersandmedia/ht/TAE.htm

 

10x TAE buffer (10x Tris-acetate-EDTA)

 TAE buffer is typically used for agarose DNA electrophoresis.

Materials To prepare 1L of 10x solution,

you need:

48.5 g Tris 11.4 mL glacial acetic acid

20 mL 0.5M EDTA (pH 8.0) Procedure Dissolve Tris in about 800 mL of deionized water.

Add acetic acid and EDTA.

Add deionized water to 1L. Store at room temperature.

Dilute stock solution 10:1 to make a 1x working solution. 1x buffer will contain 40 mM Tris, 20 mM acetic acid and 1 mM EDTA.